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产品名称:

人源细胞系

产品型号: 杭州 产品时间: 2019-01-11
人源细胞系是生物体结构和功能的基本单位,细胞的特殊性决定了个体的特殊性,因此,对细胞的深入研究是揭开生命奥秘、改造生命和征服疾病的关键。细胞生物学已经成为当代生物科学中发展较快的一门*学科,是生物、农学、医学、畜牧、水产和许多生物相关专业的一门必修课程。50年代以来诺贝尔生理与医学奖大都授予了从事细胞生物学研究的科学家。

产品概述

人源细胞系500万彩票接收后的操作流程与注意事项:

500万彩票1)TIEBIXIBAOSHENGZHANGHUANMAN;SHIDANGTIGAOXUEQINGNONGDU(ZUIGAOBUNENGCHAOGUO20%),HUOKEGENJUGAIXIBAOSHENGZHANGTEXINGSHENGZHANGMIDU,KAOLVYIMEIXIAOHUAHOU,ZHUANYIDAOXINDEPEIYANGPINGJIXUPEIYANG

2)RUGUORENYUANXIBAOXIWEITIEBIXIBAO,ERSHOUDAOSHICHENGXUANFUHUOZHEBUFENXUANFUDEZHUANGTAI,QINGJIANGXUANFUDEXIBAOJISHILIXIN,JIA15%XUEQINGDEWANQUANPEIYANGJIDAOXINDEPEIYANGMIN/PINGJIXUPEIYANG3TIAN;TONGSHIYUANPEIYANGPINGZHONGSHENGXIADETIEBIXIBAOGENGHUANWEI15%XUEQINGDEWANQUANPEIYANGJIPEIYANG3TIAN。3TIANHOURUOYUANPINGHUOZHEXINPINGZHONGDEXIBAODOUMEIYOUCHUXIANZENGZHIERSHIJIXUTUOLUOSIWANG,QINGJISHILIANXISHIYANSHIJISHURENYUANHUIGENJINJIEJUE

3)SHENGZHANGBUJUN:TIEBIXIBAORUOCHUXIANFENBUBUJUN,CHENGDAOZHUANGSHENGZHANG,KEJIANGXIBAOJINXINGXIAOHUA,ZHONGXUANDASANXIBAO,JIARUXINXIANPEIYANGJIJINXINGPEIYANG

500万彩票RENYUANXIBAOXICHANGGUIPEIYANGCHUANDAILIUCHENG(QINGYANGEZUNZHAOWUJUNCAOZUO)

500万彩票(1)XICHUYUANPEIYANGPINGZHONGDEPEIYANGJI,PBSHUANCHONGYERUNXIXIBAOLIANGCI,JIA2-3ml 0.25%YIMEIJINXINGXIAOHUAXIBAO(ZHUYIBAWOXIAOHUASHIJIAN,TONGCHANGKONGZHIZAI1-2min)

500万彩票(2)ZHUYIPEIYANGJIPHZHIBIANHUAQINGKUANG,DINGQIHUANYE(MEIZHOU2-3CI),DAIXIBAOMIDUDADAO80%YIHOUZHONGFU1XIANGCAOZUOHUOZHEDONGCUN

(3)QUBUFENXIBAOXUANYEZHUANYIDAOXINDEPEIYANGMIN/PINGZHONG,TIANJIASHIDANGDEWANQUANPEIYANGJI,BAXIBAOXUANYEDAYUN,YUPEIYANGXIANGZHONGPEIYANG

(4)JINGXIAGUANCHAXIAOHUAQINGKUANG,ZAIHS505.TRENLINBAJIECHENGXIANWEIYANGXIBAOSHENGZHANGTEXINGBIANYUANSUOXIAO,TIEBISONGDONGSHI(BUJIANYIXIAOHUADAOXIBAOPIAOFU)QUDIAOYIMEI,JIA6-8mlWANQUANPEIYANGJI,QINGQINGCHUIDAXIBAOCENG,JINLIANGBAXIBAOCENGCHUILUO,CHUISAN。

人源细胞系产品详细介绍:

CHANPINMINGCHENG:JVM-2

XIBAOXINGTAI:LINBAMUXIBAOYANG

ZUZHILAIYUAN:LINBA

CHUANDAIQINGKUANG:P3-P5  

细胞数量:1~3*106细胞/25cm2培养瓶

500万彩票PEIYANGTIAOJIAN:RPMI1640(w/oHepes)10%FBS

500万彩票CHUANDAIFANGFA:1:2~1:4CHUAN,2-3TIAN1CI

500万彩票SHENGZHANGTIAOJIAN:95%KONGQI+5%ERYANGHUATAN 37SHESHIDU

SHENGZHANGZHUANGTAI:TIEBISHENGZHANG

CUNCHUTIAOJIAN:90%FBS+10%DMSO

人源细胞系对于贴壁细胞,若细胞漂浮:培养瓶不开封,用75%酒精擦拭后平躺置于培养箱内。次日观察,如细胞大部分又贴回瓶底,表明细胞活力正常,剩余漂浮的细胞可以去掉,换成新鲜的培养基;如细胞还不贴壁,将细胞离心收集移到新的培养瓶中,原培养瓶加部分新鲜培养液继续培养,中间注意观察,我们的技术人员会一直跟踪指导,直到问题解决。对于悬浮细胞:收到细胞后,将细胞全部离心,去掉旧的培养基,换成新鲜的培养基继续培养。

人源细胞系特别注意:(如使用公共实验室或者初次接触,建议添加双抗培养)

(1)TIEBIXIBAOSHOUDAODANGTIANYOUSHAOLIANGHUOMEIYOUPIAOHUDEXIBAOKEYIDANGTIANHUANYE,YINWEILUTUDIANBOZAOCHENGDALIANGPIAOHUDEQINGKUANGSHI,QINGJIANGXIBAOHUANYEHOUFANGZHIPEIYANGXIANGFUYUDAODIERTIANZAIZUOXIAOHUACHUANDAI,QINGYOUXIANXUANZEZHIJING6cmDEPEIYANGMINJINXINGCHUANDAIPEIYANG

(2)SHOUDAOXIBAOHOUQINGJINKUAIGENGHUANWEIHAN10%XUEQINGDEXINXIANPEIYANGJI,RUYINTESHUQINGKUANGXUYAOJIXUSHIYONGYUANPING,QINGZAIYUANPINGPEIYANGJIZHONGEWAITIANJIA10%DEXUEQING,(YUANPINGPEIYANGJIDEJIXUSHIYONGSHIJIANZUIZHANGBUYICHAOGUO72XIAOSHI)

(3)RUQIANSHOUSHICHUXIANPEIYANGPINGBIPOLIE,LOUYEDENGQINGKUANGQINGJISHIZUOHAOZHAOPIANJILUBINGLIANXISUERJISHURENYUAN

 

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